Synaptosomal proteins and axoplasmic flow: Fractionation by SDS polyacrylamide gel electrophoresis

SDS Polyacrylamide Gel Electrophoresis of Proteins.

Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes. The amount of SDS bound by a protein, and so the charge on the complex, is roughly proportional to its size. Common...

Renaturation of telomere-binding proteins after the fractionation by SDS-polyacrylamide gel electrophoresis

A simple method for identification and characterization of telomere-binding proteins is described in this article. After Sodium Dodecyl Sulphate-Polyacrylamide gel electrophoresis (SDS-PAGE), proteins are eluted, renatured and used for retardation analysis with labelled oligonucleotides corresponding to human and plant of telomeric sequences. We show here that this method is efficient to recove...

Proteins – structure, properties and their separation by SDS- polyacrylamide gel electrophoresis

This paper deals with three basic aspects: 1) basics of protein structure in relation to physicochemical characterization, protein stability, importance of disulfide bond and their structure; 2) basic principles involved in polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE and the rationale behind the use of disc electrophoresis; and 3) detailed protocol involved in SDS-PAGE in terms of re...

Fractionation of heparin-derived oligosaccharides by gradient polyacrylamide-gel electrophoresis.

Heparin-derived oligosaccharides, prepared by using flavobacterial heparinase, having a high degree of heterogeneity (sequence variability) were resolved into sharp well-defined bands by using polyacrylamide gel electrophoresis (PAGE). The use of a stacking gel and a high-density-pore-gradient resolving gel was primarily responsible for the success of this separation. Low-Mr standards of known ...

Unpredictable behaviour of mucins in SDS/polyacrylamide-gel electrophoresis.